Composite

Part:BBa_K2671420:Design

Designed by: Oliver Hild Walett, Johan Larsson   Group: iGEM18_Linkoping_Sweden   (2018-09-11)


TetR-TetP-GroES


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The GroES sequence was ran through IDTs codon optimization tool, because parts of it had high GC content and inverted repeats. The sequence will not give any result in BLASTn.


Source

E.coli chromosome 1

References